The isolated temperature-sensitive mutants were backcrossed to pcp1 + -mRFP-kan r to ensure proper integration and to remove potential off-site mutations.
To analyse the essential functions of Pcp1 in cell division, we isolated temperature-sensitive (ts) mutants of pcp1 + using an error-prone PCR method.
As pla1 and rna15 are essential for cell growth, we isolated temperature-sensitive (ts) mutants of these two genes and used a viable pab2 deletion mutant in subsequent experiments.
This makes it more difficult to screen a mutagenised library and, in turn, to isolate temperature sensitive mutants.
If no ionic strength effect is identified on Sr/Ca and Ca/Ca ratios, both may be used as potential proxies for past SST and to isolate temperature and salinity effects on O/O ratio in inorganic calcium carbonates.
Analysis of covariance of infectivity in the additional replicate experiment, testing effects of pathogen isolate, temperature (10 vs 20°C) and infectivity in the main experiment (covariate); all other interactions (P > 0.8) removed from model.
Several of these early screens focused on isolating temperature-sensitive mutations of developmental processes (Hirsh and Vanderslice 1976; Miwa et al. 1980; Schierenberg et al. 1980; Wood et al. 1980; Cassada et al. 1981; Kemphues et al. 1988).
Unlike the pseudohomogeneous model where the maximum exit temperature cannot exceed the adiabatic temperature rise (Pem → ∞), the heterogeneous model can have isolated high temperature branches that exist when particle Lewis number is less than unity.
It is known that a deep dehydroxylation of silica occurs in this temperature range [38], and practically all OH-groups appear to be isolated at temperature over 400 °C.
In addition, it is found that isolated high temperature branches can exist in the adiabatic reactor when Lep < 1.
