Sentence examples for isolated taste from inspiring English sources

We isolated taste buds and cells from lingual vallate epithelium of PLCβ2-GFP mice, loaded them with the Ca2+-sensitive dye Fura-2, and functionally imaged for changes of cellular [Ca2+].

To examine this larger population of cells, we isolated taste cells from TRPM5-GFP expressing mice.

Isolated taste cells that responded to OXT were neither Receptor (Type II) cells nor Presynaptic (Type III) cells, consistent with the molecular expression data.

To identify taste bud-associated genes, we compared gene expression between isolated taste buds and lingual epithelium lacking taste buds.

To independently verify the cell-type that expresses OXTR, we used RT-PCR on isolated taste cells.

Total RNA was extracted from multiple isolated taste buds from the circumvallate papillae, non-gustatory lingual epithelium, and the brain (cerebellum) using Trizol (Invitrogen Corporation, Carlsbad, CA) according to the manufacturer's protocol.

LCM was used to isolate taste tissue from macaque and human samples.

While we removed the taste buds from the surrounding epithelium to isolate taste bud RNA for the RT-PCR analysis, it is possible that some surrounding epithelial cells were also collected.

Real-time RT-PCR experiments were performed on total RNA isolated from taste buds of foliate papillae and nontaste epithelial tissue devoid of taste cells as described previously 36.

RNA was isolated from taste tissue (vallate papillae, foliate papillae, fungiform papillae) and from other rat tissues (heart, trachea, bronchi, and lungs, nasal respiratory epithlium, larynx, spleen, liver, gut, stomach, testicle, brain) using TRIzol Reagent (Invitrogen, life technologies).

The limited abundance and difficulty in isolating primate taste buds devoid of contaminating lingual epithelial cells that has hindered molecular analysis of primate taste cell gene expression was overcome by using the macaque as a source of tissue and LCM as the method of tissue collection.