Sentence examples for isolated stimulated from inspiring English sources
To investigate this, human primary T cells were isolated, stimulated with agonist antibodies to the TCR complex and β-catenin expression was analysed by Western blotting.
Gastric epithelial cells and myeloid-derived suppressor cells (MDSC) were isolated, stimulated and/or cultured for Th22 cell function assays.
Primary rat adipocytes expressing wild-type or Ser358Ala HA–SIK2 were isolated, stimulated and homogenized in ice-cold homogenization buffer containing 30 mM Tris/HCl (pH 7.5), 1 mM EGTA, 1 mM EDTA, 0.25 mM sodium orthovanadate, 10 mM sodium-2-glycerophosphate, 10 mM sodium fluoride, 5 mM sodium pyrophosphate, 0.27 M sucrose, 1 mM DTT and complete protease inhibitor.
To verify that our CAF isolates stimulated epithelial growth, we confirmed that BPH1 cells proliferated faster in conditioned medium from CAFs, the effect being modest but significant (Fig. 1B).
Furthermore, culture supernatants from GGS associated with STSS showed no mitogenic activity towards peripheral blood mononuclear cells (PBNC), and recombinant proteins encoded by speG dys from the same bacterial isolates stimulated PBCN only weakly in a recent study [ 54].
Total RNA was isolated from stimulated (tumor necrosis factor-alpha (TNF-α) or CXCL16) or non-stimulated HMVECs and EPCs.
Maternal splenocytes were isolated and stimulated for cytokine production.
Lymphocytes were isolated and stimulated with lipopolysaccharide (LPS; Escherichia coli, 26 B6).
(C) MEFs from wild type, CARMA3−/− (Card10−/−), and Bcl10−/− embryos were isolated and stimulated with doxorubicin (1.5 μg/mL) for indicated periods.
Splenic CD4 T cells of NZB mice were isolated and stimulated by co-culture of T cells with individual band 3 peptides.
Adult rat ventricular myocytes were isolated and stimulated to contract at 0.5 Hz, and mechanical and intracellular Ca2+ properties were evaluated using an IonOptix Myocam system.
