A total of 16 B. quintana isolates spanning over 60 years and three continents were characterized.
A total of 54 unique sequences were in the final set analyzed comprising wild type isolates spanning 1945 2011 and 11 attenuated vaccine strains.
This demonstrated the presence of pKPC_UVA01 in 41% (15/37) of the KPC- Kp isolates, spanning 10 STs over a 3.5-year period (Fig. 1; see Table S2 in the supplemental material).
North America is well represented in this study with 273 isolates spanning 44 MLVA genotypes (A.Br.WNA plus isolates from other sub-lineages, Figure 3B).
The second contains all the O 25b and multidrug-resistant isolates spanning an 8-year period and from the United Kingdom and India and shows very little diversity, in concordance with earlier data from our group (Clark et al. 2012).
Sampson, unpublished results) and a recent study by Talarico et al. [ 31] found sequence variation for PE_PGRS33 (Rv1818c) in 68% of clinical isolates spanning all three M. tuberculosis principal genetic groups [ 32].
The dataset used, which comprises 515 alleles found in more than 831 isolates spanning a great taxonomical host range, reflects a substantial sampling diversity of WSP and allowed grouping of recently diverged WSP proteins and HVR motifs.
The 515 wsp alleles were found in overall 831 Wolbachia isolates, spanning all major arthropod host taxa and continents (see additional files 5 and 1 for a summary and for the whole dataset, respectively).
The isolates included those collected from animals, humans, and other sources (i.e., industrial sites associated with anthrax outbreaks); the isolates span at least 58 years (1939 1997).
In contrast, all pulsotypes including >5 isolates were found in multiple geographic regions, and 3 of the 6 pulsotypes comprising >8 isolates spanned all 6 geographic regions (Table 2).
Four sequential BAC clones, 184A3, 198E6, 201K4, and 174E15 were isolated to span the area from the AU171175 marker to the pc locus.
