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One positive clone of 2570 bp was isolated, sequence analysis revealed that it contained a poly A tail and an open reading frame of 1488 bp, which was latter found to encode amino acids 89 to 583).
However, only a limited number of biologically interesting features can be revealed from an isolated sequence.
The functional annotation of GluRS, without recourse to experiments, performed in this work, demonstrates the inherent and unique advantages of using whole genome over isolated sequence databases.
Most of the existing tuatara mRNA sequences are only partial coding sequences, and in many cases our sequences only overlapped with a portion of this previously isolated sequence.
In database, a previously isolated sequence [GenBank: BT011697] is different from both the prediction of At1g56140 in TAIR8 and the sequence from this report, losing the sequence from exon 6 to exon 23 and part of exon 24, resulting in a much smaller protein with 184 aa compared to the predicted protein of 1,032 aa.
Phylogenetic analyses confirmed that the isolated sequence showed a high degree of similarity with other already isolated HQT sequences [ 25, 41] and with HCTs from globe artichoke [ 32], coffee [ 41], tobacco and Arabidopsis [ 24] (Fig. 3).
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Last year, the U.S. Supreme Court ruled in the high-profile case Association for Molecular Pathology v. Myriad Genetics that isolated sequences of human DNA are not patentable.
"Anna Span's win was a big moment," says Chris Ratcliffe, head of Television X. "The BBFC began with a position of complete prohibition, and now squirting is allowed in isolated sequences.
The pET-MstnPP plasmid was isolated, sequenced and used to transform E. coli BL21 DE3) cells for protein expression.
The DENV2 strain ZS01/01 (GenBank accession no. EF051521.1) has been previously isolated, sequence-verified, and preserved in our laboratory.
A 300 bp band was isolated, sequenced and confirmed to be the 5' DFP-4 sequences.
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