Sentence examples for isolated reverse from inspiring English sources
Total RNA was isolated, reverse transcribed, and real-time PCR performed.
RNA was isolated, reverse transcribed, labeled and hybridized to the arrays and the ratios calculated.
Total RNA was isolated, reverse transcribed to cDNA and the obtained cDNAs were PCR amplified with the corresponding primers.
Messenger RNA was isolated, reverse transcribed, and analyzed in real time with the StepOne Plus (Applied Biosystems, Carlsbad, CA, USA) and run in duplicate.
RNA was isolated, reverse transcribed and undergone real-time PCR.
Subsequently, total RNA was isolated, reverse transcribed and used for sqRT-PCR with primers specific for G6PD, PEG10, c-MYC (endogenous) and ectopic c-MYC.
At 48 hrs post-transfection, total RNA was isolated, reverse-transcribed and quantified by RT-PCR using the Taqman probes against ENT4 as described above.
At 72 hours post transfection, RNA was isolated, reversed transcribed and used for RT-PCR analysis.
Total RNA from two individuals per strain was isolated, reversed transcribed, and subsequently labelled according to a recently developed protocol (adapted from Xiang et al., 2002), which requires an input of only 1 μg total RNA.
The primary method of identifying miRNA genes has been to isolate, reverse transcribe, clone, and sequence small RNA molecules [ 14- 16].
Only one serum isolate reversed morphological appearance by showing multiple punctuate pattern of inclusions with extracellular particles on the third passage.
