Exact(1)
Apple went to great lengths to emphasize just how segmented its fingerprint scanner was from the rest of the hardware, and how isolated (read: protected from hackers) the data that it gathered was.
Similar(59)
The notions of contig and singleton are straightforward for perfect assemblies: a contig is any sequence produced by two or more overlapping reads, while singletons are the remaining isolated reads.
After the data-cleansing steps were completed each newly sequenced isolate read set contained 11,400,000 paired reads.
First, we can modify it to maximize the number of paths (haplotypes) that consist of a single read, isolating reads that are likely to contain errors.
Although step 4b) is designed to isolate reads that likely contain errors so they can be ignored (see below), many sequencing technologies induce frequent errors that are easy to identify.
Similarly for rifampin, 20 (91%) correlated, but one isolate, read as susceptible for rifampin by Bactec MGIT, had a MIC indicative of resistance (>16 μg/ml).
In both isolates, read depth was evenly distributed along the entire sequence of Chr31, arguing against region-specific amplification (Fig. 2).
For each of the three isolates' read dataset, the number of base pairs in the Muguga reference genome with no read coverage determined from the bam files, using a custom script.
The request for isolates read as follows: "To determine the US level of A. fumigatus azole resistance, we are requesting submission of: 1) All new or stored isolates of Aspergillus fumigatus from US collections; 2) Any new isolates of A. fumigatus regardless of clinical relevance".
That is, rather than picking and choosing isolated verses, read whole books of the new testament at a time.
For each clinical isolate, reads were mapped using Burrows-Wheeler Aligner (BWA) version 0.7.5a against pKPC_UVA01 and pKPC_UVA02 (details in supplemental material).
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Justyna Jupowicz-Kozak
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