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Another striking difference was that strains belonging to Actinobacteria were isolated only by the APD isolation method.
Pure epithelial cervical cells could reproducibly be isolated only by outgrowth from tissue explants as other previously described cell isolation methods did not lead to a pure and viable primary cell population in our hands.
They can be isolated only by a harsh additional collagenase treatment of the dermis 29.
S. pyogenes, Mycobacterium tuberculosis, and P. aeruginosa were isolated only by culture (individual PCR assays for these organisms were not available), and M. pneumoniae was detected by PCR in 1 child.
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One was part of ST53 together with 5 isolates from Denmark and Sweden, and the remaining were distinct but deviated from neighbouring Danish isolates only by 1 to 4 substitutions in the 4287-bp concatenated sequence.
But by isolating only power plants and refineries, the agency also seemed to signal that for now, at least, it will go after only big industrial sources.
MICs to oxacillin were determined for all isolates and to vancomycin for MRSA isolates only; MICs were determined by Etest (AB Biodisk, Slona, Sweden).
They were isolated, governed only by the ethos of their small-town attendees and their traditional values.
There's also a high level of "double segregation," Orfield said in an interview, as students are increasingly isolated not only by race, but also by income: the typical black or Latino student in New York state attends a school with twice as many low-income students as their white peers.
PFGE analysis of the North American strains indicated that all three were closely related as two of three had identical PFGE profiles with the remaining isolate differing only by a single band position.
So, to get at the question of what kinds of traits and processes underlie speciation, it is preferable to study species pairs or complexes that are recently diverged and isolated by only one or a few traits [2].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com