That isolate, ON-2010, was recovered in June 2010 from a 10-month-old boy who had returned from the Philippines 2 days earlier.
The transformants (ctcH+, ctcJ+, and ctcH ctcJ+) were isolated on ISP4 plates under apramycin and nalidixic acid selection, cloned on MS plates, and verified with PCR and a transcription analysis.
These microbes were originally isolated on MEA+1.5S, MEA+3.0S, MEA+3.0 NaCl, 0.1% Potato Dextrose Agar, and Nutrient Agar, suggesting tolerance for a broad range of nutrient levels and salinity.
Mature gametocytes of P. falciparum (NF54 strain) were produced in an automated static culture system [26], isolated on 63% Percoll [no. 17-0891-01, GE Healthcare, UppSwedenSweden] and stored at −70°C until used.
The remaining half of each of the tissue samples isolated on 3 dpi and 14 dpi from the mice infected with the APMV serotypes was fixed and embedded in paraffin, and tissue sections were prepared.
DENV-3 was isolated on C6/36 cells (Aedes albopictus).
Peripheral blood mononuclear cells (PBMC) were isolated on D40.
The latter two were however also isolated on FD4 and/or FD2 media.
Lb. paracasei was the dominant species (72 isolated on MRS and 16 on M17 agar plates), followed by Sc. thermophilus (50 isolated on M17 agar) and Ln.
lactis isolates amongst 13 were found on MRS agar plates and about 14% of all rod-shaped isolates were isolated on M17 agar plates.
