Sentence examples for isolated nucleus from inspiring English sources

In this paper, we use a Finite Element Method to solve a Time Dependent Multi-ion Transport and Reaction Model (FEM-TD-MITReM) to report on the growth of an isolated nucleus.

The isolated nucleus pulposus cells and articular chondrocytes were cultured in Dulbecco's modified Eagle medium: Nutrient Mixture F-12, 1 1 Mixture (DMEM/F-12) (Wako Pure Chemical Industries Ltd., Osaka, Japan), containing 10% fetal bovine serum (FBS; Gibco Invitrogen), 100 U/mL penicillin (Gibco Invitrogen) and 100 μg/mL streptomycin (Gibco Invitrogen), at 37°C in 5% CO2 humidified atmosphere.

B Low magnification EM micrograph of the lamella of isolated nuclei.

Open image in new window Fig. 4 Cryo-ultramicrotomy analysis of Hela cells and isolated nuclei.

Objective: To compare the oocyte-activating ability of whole human round spermatids and their isolated nuclei.

During scATAC-seq, isolated nuclei are processed by Tn5 tagmentation, which inserts adapters into nucleosome-free regions [72, 73].

Open image in new window Fig. 1 EM micrographs of conventional chemically fixed ultrathin sections of Hela cells and isolated nuclei.

For the DNA content estimation of the isolated nuclei the Partec CyFlow ML flow cytometer with green solid state laser (532 nm wavelength and 150 mW) and band-pass 590 nm optical filter was used.

Western blot analysis revealed negligible contamination of isolated nuclei with the abundant cytoplasmic protein, α-tubulin (Figure 3a), and low contamination of the cytoplasmic fraction with the soluble nuclear protein HDAC-1 (Figure 3b).

Engineered vectors containing capsid variable regions (VR) VII & IX from AAV8 in an AAV2 backbone mediated near AAV8-like transduction in mouse liver, with higher numbers of chimeric genomes detected in whole liver cells and isolated nuclei.

Only freshly isolated nuclei were used in each experiment.