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Furthermore, none of these studies determined whether the mesenchymal cells isolated maintained multipotential capacity in vivo.
NMG and CRC isolated, maintained and stored fungal samples.
Astrocytes from both normal (ages 59 ± 13) and glaucomatous (ages 69 ± 13) eyes with mild, moderate or advanced (1 donor) axon loss were isolated, maintained and processed for analysis in a strictly similar manner so that cultivation-induced differences on gene transcription between cell lines would be minimized.
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The RT-LAMP results obtained with known CTV isolates maintained in screen house of CCRI, Nagpur were validated using field samples and thereafter it was further confirmed by conventional RT-PCR (reveres transcription-polymerase chain reaction) assay.
Steenken and Gardner [3] described the media used at Trudeau during the time, until 1922, in which the isolate maintained its virulence for rabbits and guinea pigs.
The HPAIV strain A/Egret/Egypt/01/2006 (H5N1) was obtained from Dr. Daniel Perez and the HPAIV strain H5N2 strain (A/Pennsylvania/8/3/1983) was a field isolate maintained in our virus repository.
From the original HpSVd-hop isolate maintained in five hop plants, 22 mutation positions were identified in 67 cDNA clones (5 from the inoculum and 62 from the progeny).
Increased wrinkling occurred with a B. subtilis NCIB3610 isolate maintained by us and also with an NCIB3610 isolate obtained from the Losick laboratory (referred to here as NCIB3610-H).
However, 5 patients colonized with levofloxacin-resistant S. aureus isolates maintained persistent nares and/or stool cultures during therapy with this agent.
Recent re-sequencing experiments have also been able to characterize the variations between the H37Rv isolates maintained at multiple laboratories (4).
Vero cell adapted PPRV Coimbatore CBEE) field isolate, maintained in the Department of Veterinary Microbiology, Madras Veterinary College, Chennai 600 007, was used throughout this study to standardize the real-time RT-PCR assay.
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