Sentence examples for isolated in duplicate from inspiring English sources

Total RNA was isolated in duplicate from the parental RH strain, from mutant 88A5, or from a genetically rescued 88A5 clone (88A5-C) all cultured for 24 h at the permissive (34°C) or non-permissive (40°C) temperatures.

Mitochondria were isolated in duplicate independent samples from each of the brain tumor cell lines for sequence comparison.

36M2 cells were treated with carboplatin 100 μM or vehicle-control for 24 hrs, and RNA was isolated in duplicate at selected time points (as described in Material and Methods) from initiation of exposure until early apoptotic changes became apparent.

The experiments were carried out with groups of five rats for each isolate, in duplicate and on two different days.

Minimum inhibitory concentrations were determined for each isolate in duplicate by microdilution techniques according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI).

The mutation frequency (nucleotides/site/year) of four natural HpSVd isolates grown in duplicates in hop plants were calculated.

The invasiveness of each L. monocytogenes isolate was analysed in quadruplicate (2 independent invasion assays, with each isolate analysed in duplicate in each assay).

Similarly, for two isolates sequenced in duplicate in this study, one of serotype 19A and the other 19F, the pairs of consensus sequences were only distinguished by two and three polymorphic sites, respectively.

Total RNA and mRNA were isolated and measured by spectrophotometry; total protein was measured by BCA assay (measured in duplicate, isolated from 10 cells; mean ± SD).

Freshly isolated PBMCs were plated in duplicate at 50,000 200,000 cells per well and incubated with peptide pools at a final concentration of 2 µg/ml per peptide for 16 18 hours at 37°C in a 5% CO2 atmosphere.

To test reproducibility, fifteen isolates were tested in duplicate, two in triplicate and one quadruplicate.