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Nuclei were isolated in batches of 24 for amplification.
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68,000 and 45,000 of 7BS and 7BL arms, respectively, corresponding to 50 ng of DNA, were isolated in several batches.
Hemocytes were isolated from 750 larvae in batches of 50 as described [19].
Our annotation shows slight differences with those of Liu et al. Total RNA was isolated from zebrafish embryos at 51 hpf in batches of 50 80 embryos, using a QIAGEN RNeasy Fibrous Tissue kit.
RNA was isolated from batches of ∼106 cells using the RNeasy Mini Kit (Qiagen; www.qiagen.com).
Hemocytes were isolated from a total of 300 third instar larvae of each genotype in batches of fifty animals.
ascorbic acid, lysates were stored at −20°C.Freshly isolated islets from P15.5 pregnant female mice were incubated for 1 h in batches of 50 at 37°C.
To avoid overcrowding the pan, fry in batches of eight.
Eggs are deposited in batches of around four.
RNA samples were processed in batches of 96 samples.
We sequenced clones in batches of 10.
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