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In the resulting engineered cells, isolated functional measurement of the remaining allele carrying the rare mutation of interest is possible.
Myocardial tissue engineering combines isolated functional cardiomyocytes and a biodegradable or nondegradable biomaterial to repair diseased heart muscle.
The formation of functional group dimers may decrease the solubility of CO2 because of their weaker interactions with CO2 than isolated functional groups.
To overcome this limitation, we constructed a library of FHA1 variants by mutagenic PCR and isolated functional variants after three rounds of affinity selection with its pT peptide ligand.
Therefore, tumor suppressors may theoretically be any of a variety of cellular factor, or even isolated functional parts of gene expression products.
Analysis of an entire disease-related system might provide insight to the molecular etiology of the disease that would not emerge from isolated functional studies of single genes.
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To overcome this situation, we recently established a method for isolating functional single cells from environments using a micromanipulator (Functional single-cell (FSC) isolation), which we applied in studying denitrifying bacteria in rice paddy soil ([Ashida et al. 2010]).
Slice preparations isolate functional networks, permitting single unit recording under visual control, and the use of fluorescent indicators.
This knock-down strategy, together with available methods to isolate functional intrabodies, should allow the large-scale investigation of intracellular protein networks.
Following binding selections to isolate functional clones, DNA sequencing was used to determine the wild-type/mutant ratios at each varied position, and these ratios were used to assess the contributions of each side-chain to antigen binding.
However, cells transduced with islet-specific TCRs were less responsive to cognate antigen than viral-specific TCRs, and in some cases, required additional methods to isolate functional antigen-specific Tregs.
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