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Four days after primary insult, knee joints were isolated for assessment of PG synthesis and PG content.
The hypothalamus was again isolated for assessment of differential gene expression, this time using a smaller QPCR array of 48 genes.
Thoracic aortas were isolated for assessment of either VEGF signaling or vascular reactivity as described previously (Wheat et al. 2011; Conklin et al. 2009) with some modifications.
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The descending aorta from 14-week +/ Col3a1▵ (3 males:3 females) and +/+ mice (2 males:3 females) was isolated for functional assessment in vitro, as described previously.
The mice were then euthanized with CO2 and the spleen cells were isolated for T cell assessment.
Therefore, after treatment with 100 and 200 nM rotenone, nuclear and cytosolic cell fractions were isolated for MEF2D protein assessment.
Four days after IL-1 injection, patellae were isolated for proteoglycan synthesis measurement by SO42- incorporation (n = 92) or whole knee joints were isolated for histological assessment of proteoglycan content of patellar and tibial cartilage (n = 24).
"We have been isolated for so long.
You can be isolated for it.
Therefore, no protein spots were isolated for MS. This prospective cohort feasibility study recruited 11 consecutive patients following successful resuscitation from cardiac arrest to determine the feasibility of performing a proteomic assessment of patient sera.
Hence, bowel sections were isolated for immunoblotting.
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