Sentence examples for isolated fibroblasts from inspiring English sources

With this in mind, we have isolated fibroblasts from different dermal regions to investigate age-associated changes in their DNA methylation profiles in comparison to MSC.

To compare the differential effects of CAFs and NAFs on breast tumorigenesis, we isolated fibroblasts of human breast cancer tissue and adjacent normal breast tissue from the same patients.

We could detect higher Mx1 expression levels in isolated fibroblasts at both mRNA and protein levels.

In isolated fibroblasts, surfactant causes a decrease of DNA-synthesis [ 9].

In contrast, optimized single cell culture conditions for isolated fibroblasts may protect the cells in a more artificial manner.

Besides splenocytes, we also isolated fibroblasts from the lungs of the Oct4 -GFP neonates (Yau et al., 2011).

Taken together, the results indicate that the isolated fibroblast populations were substantially pure (>95%) and not contaminated with epithelial, myoepithelial, endothelial, inflammatory, or pericyte cell populations.

To isolate fibroblasts [ 35, 36], epithelial tissue was washed twice in PBS and cut into 1 2 mm pieces.

The dermis was then cut into small pieces and digested with 0.1% collagenase type I (Gibco, USA) for 4 hours at 37°C to isolate fibroblasts.

Although the role of TGF-β in cancer cells and lung fibroblasts has been investigated extensively, TGF-β function in CAFs remains largely unknown due to technical hurdles in isolating fibroblasts from lung cancer tissues.

The dermis was cut into small pieces and incubated at 37°C for 20 hours in a collagenase H solution (0.125 U/mL, Roche, Laval, QC, Canada) in order to isolate fibroblasts from the connective tissue.