Researchers have already come up with ways to isolate engineered species.
One of the isolated variants cleaved the Gln-containing peptide substrate more efficiently in vitro, proving the efficiency of our method in isolating engineered proteases with desired substrate selectivity.
To understand the contributions of structural and nonstructural genes to HCV replication potential and infectivity, we have characterized intragenotypic recombinant genotype 2a viruses with different portions of the J6 isolate engineered into the JFH1 infectious clone.
Similar to Gardner and colleagues, they used combinations of lac operators and RBS of varying strength, but instead of testing each construct separately, they used genetic selection to isolate engineered circuits that confer regulatory and phenotypic behaviour similar to wild-type λ phage.
Exploiting a natural phenomenon observed in tumors, plasmid DNA encoding for a soluble ligand to NKG2D (sRAE-1γ) was isolated and engineered into a plasmid expression system.
We have characterized the IgG form of a previously isolated and engineered single-chain Fv (scFv), named RR2r3s4-1, that binds to human PSGL-1.
The large-scale production of oligosaccharides using either glycosyltransferases isolated from engineered microorganisms or whole cells as an enzyme source could promote a new era in the field of carbohydrate synthesis.
The adoptive transfer of T cells isolated or engineered to have specificity for diseased cells represents an ideal approach for the targeted therapy of human viral and malignant diseases.
The ease with which plasmids are isolated, genetically engineered, and re-introduced has led to their near ubiquitous use in synthetic biology, despite the potential consequences of variable copy number.
