Prostate bone metastases paraffin-embedded sections, isolated from consenting patients, were mounted on MirrIR slides and deparaffinised with Citroclear followed by 20 min acetone treatment before air drying.
Inclusion criteria were: informed consent, isolated CABG surgery, age >18 and < 75 years, Hct > 36% and bodyweight > 70 kg.
Peripheral blood lymphocytes (PBL) were isolated after informed consent from PBMC of healthy donors, by depleting CD14+ cells using CD14 Microbeads (Miltenyi Biotec).
Non-transformed cells included human PBL and skin fibroblasts isolated from six consenting healthy donors and human umbilical vein endothelial cells (HUVEC, PromoCell, Heidelberg, Germany, EU).
Primary CLL cells were collected from patients' peripheral bloods after informed consent, and isolated by Ficoll/Hypaque density-gradient centrifugation as previously described [10].
Skin lavage fluids and serum samples were isolated after written informed consent had been obtained.
Human islets (obtained with ethical approval and clinical consent) were isolated from pancreases of 46 nondiabetic donors and 10 donors with T2D.
With informed patient consent, ascitic fluid isolated ovarian cancer cells, cell lines and ovarian cancer spheres were co-cultivated with human platelets.
The human knee chondrocytes were isolated from autopsy donors (no consent was needed) that were graded macroscopically according to a modified Outerbridge scale [ 14, 15].
After obtaining written consent, MSCs were isolated from at least four different human umbilical cords obtained from full-term infants 38-400 weeks) by explant culture, expanded and cryopreserved until the start of the experiment as described elsewhere [ 3, 29].
After written informed consent, DNA was isolated from peripheral blood samples of 38 individuals who developed CRC before the age of 45 years and had negative family history of the disease (or other HNPCC-associated cancers; Table 1), using the salt chloroform extraction method (Müllenbach et al, 1989).
