The higher turnover for the bovine isolate compared with the human isolate might reflect a more recent adaptation to this environment.
In concordance with the in vitro data, genes in ΦSA3usa were more highly expressed during lung infection in the USA300 isolate compared with the USA400 strain.
The phenotypic results of susceptibility tests of GN isolates compared with the qPCR results showed discrepancies in ESβL detection for six samples.
Phylogenetic analysis showed an 87.3% 98.2% aa sequence identity of env genes in all ALV isolates compared with the HPRS-103 strain (5 ).
Role category analysis of genes considered to be part of the shared core USA300/USA400 genomes demonstrated an overall higher gene-expression profile in most functional categories in the USA300 isolate compared with the USA400 isolate.
Genes that had significantly higher expression in the USA300 isolate compared with the USA400 strain included: immunoglobulin G binding protein A precursor (SAUSA300_0113), fibrinogen-binding protein (SAUSA300_1055), superantigen-like protein (SAUSA300_0407) and a leukocidin family protein (SAUSA300_1975).
Specifically, supercontigs 6, 7, 8, 9, 10, 13, 14, 15, and 16 showed a greater chromosome copy number in the JEL427-P9 isolate compared with the JEL427-P39 isoland, and when compared to the average of other isolates studied to date (Rosenblum et al. 2013; Figure 2).
Despite the relatively small numbers of Acinetobacter spp. isolates compared with the other organisms, carbapenem-resistant Acinetobacter spp. were found in all ICUs at a high incidence density; as many as 69% of all isolates at 1 ICU were carbapenem resistant.
There were also a number of lipoproteins and proteases more highly expressed in the USA300 isolate compared with USA400 strain.
The degrees of antibiotic resistance for all antibiotics tested were significantly higher in the CRPA isolates, compared with CSPA isolates (Table 3).
