Sentence examples for isolated breast from inspiring English sources
For the first time, we confirmed the malignant origin for all individually isolated breast cancer CTCs.
To minimize contamination in the methylation analysis, we isolated breast cancer cells and paired normal breast epithelial cells from tissues by manual macrodissection.
In 2003, Al-Hajj et al. have identified and isolated breast CSCs from patients using the cell surface marker pattern CD44+CD24-/lowLineage-[ CD44+CD24-/lowLineage-[ CD44+CD24-/lowLineage-[ 10]
The relationship between NRP2 and BMI-1 was further confirmed using NRP2high and NRP2low sorted populations from freshly isolated breast tumours (Fig 3C).
To avoid contamination with normal tissues in the methylation analysis, we isolated breast cancer cells and paired normal breast epithelial cells from tissues by manual microdissection, following the National Cancer Institutes (Bethesda, MA, USA) protocols [ 22].
To avoid contamination with normal tissues, we isolated breast cancer cells by microdissection and compared levels of methylation with those in normal breast epithelial cells from the same patients.
Attempts to identify and isolate breast stem cells have relied upon expression of cell surface markers.
Among the different methods utilized to isolate breast CSCs some are promising but require validation with a combination of two or more methods to improve our knowledge on the cells that drive tumor growth and evolution.
We also searched for assays conducted in breast cells or with proteins isolated from breast cells.
In addition to the immunohistochemical analysis, we performed western blot analysis on primary breast fibroblasts and epithelial cells isolated from breast tissue.
Total RNA was isolated from breast cancer cell lines and homogenised breast cancer samples using the AB gene Total RNA Isolation Reagent Advanced Biotechnologies Ltd.., Epsom, Surrey, UK).
