Although the number of positively stained cells was lower in fimbriae producing rough colony strains than in the non-fimbriated Δflp1-flp2 mutant, positively stained rough colony isolates bound more IL-1β per cell than the non-fimbriated control strain.
The results demonstrated that Opc-expressing Nm isolates bound to a 100 kDa protein present in HUVEC lysates.
All of the polymyxin B-resistant RSF34 isolates bound dansyl-polymyxin B similarly to RSF34, indicating that the levels of dansyl-polymyxin B binding in these mutants are due to the heptoseless LPS and that increased polymyxin B resistance in these mutants is not due to decreased polymyxin B binding.
The majority of P. falciparum isolates bind CD36 (1– 4) yet only a fraction of infections lead to life-threatening consequences.
Previous results showed that the GI.8 BV, a clinical isolate, bound uniquely to the Le, but not the A and H antigens (Huang et al., 2005), making the BV distinct from other tested GI NoVs that bind generally the A and H antigens (Shirato et al., 2008).
In this study, we observed that 80% of clinical isolates bound to both CD36 and ICAM-1 (Table S2).
Results showed that primary isolates bound to TSn cells in a manner similar to HIV-1NL4-3 and that binding was abrogated by pretreatment with the anti-Sn mAb 7D2 or virus pretreated with sialidase (Fig. 4A).
As shown in Fig. 4A C, PA-HA from several FluA strains of various subtypes, including a human H5N1 isolate, bound to both PB11 25A and PB11 25 AT6Y, but not to PB11 25B or a scrambled biotinylated peptide.
Using a new ß-lactamase-based protein fragment complementation assay (PCA), an affibody molecule was isolated which bound a ZHER2 affibody molecule with sub-micromolar affinity, but not unrelated affibody molecules.
Compared to the crystal structure of the isolated clamp bound to DNA (Georgescu et al., 2008a) the clamp is rotated by ∼20°, resulting in an almost perpendicular orientation (∼80°) with respect to the DNA.
