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These multipotent cells were isolated and established from adult mouse testes using genetic selection, and the rate of establishment of cell lines was about 27% [ 4].
We isolated and established iPSC lines that were generated from Δ133p53-overexpressing, p53-knocked-down, control vector-transduced and untransduced fibroblasts (all cell lines established in this study are listed in Supplementary Table S1).
Primary bone marrow mesenchymal cell were isolated and established by flushing bone marrows from the tibiae, femora, and humeri of 4 week-old animals with cold DMEM using a 27-gauge needle.
GBM 8401 cells were isolated and established from a Chinese female patient with brain malignant glioma.
Rickettsiae were successfully isolated and established in Vero cell culture from the female tick.
Antibiotic-resistant colonies showing HIF-1-dependent bioluminescence were isolated and established as clones.
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To isolate and establish the mechanistic contributions of microstructural features (dendritic α-Al matrix/eutectic phases/Mg Si strengthening precipitates), alloys with various Si content/morphology, grain size level, and matrix strength were studied; the effect of secondary dendrite arm spacing was also assessed.
To isolate and establish putative canine neural stem cell (NSC) and radial progenitor cell (RPC) in monolayers, Noggin-restricted colonies were isolated and passaged on MEF seeded 4-well dishes in N2B27 media with 20 ng/mL FGF2.
Recently, several groups reported success, both in humans and in large animal models, in isolating and establishing MSCs cultures from fetal adnexa such as amniotic membrane, amniotic fluid or UC (human:[7], [8], pigs [9], horses [10] [15] and dogs [16] [18].
The other, was used to isolate and establish a carcinoma-associated fibroblast cell line.
Spoligotyping was used to genotype the MTBC isolates and establish the circulating lineages.
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