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About three hundred pairs of apical and basal cells from the two-celled proembryos were isolated and collected respectively for RNA isolation.
Chondrocytes were isolated and collected according to the procedure proposed by Malicev et al.[19], with slight modifications.
Primary, two-layer secondary, multi-layer secondary and antral follicles were mechanically isolated and collected from day 8, 12, 16, 21 22 mice as described below.
For subsequent RNA extractions, mammospheres were isolated and collected by centrifugation as per manufacturer's protocol.
Next, eGFP+ and eGFP− cells (5 × 10 cells per ml in PBS supplemented with 10% FBS) were isolated and collected using a BD FACSAria cell sorter (BD Biosciences, Sparks, MD, USA).
Cells expressing GFP were isolated and collected 48 hours later on a MoFlo cell sorter (Cytomation), and were subsequently expanded to yield stable polyclonal populations of control, CystC-expressing, or Δ14CystC-expressing cells.
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The lack of investigators' accessibility to the physical infrastructure of the cloud and the unknown location of the cloud data centers make it much harder, if not impossible, to identify, isolate and collect forensic data in the cloud.
AL co-conceived the study, collected isolates and collected antimicrobial use data.
Statistical analyses were performed to identify significant changes in resistance between years and to investigate associations between patients with resistant gonococcal isolates and collected epidemiological variables.
After 72 h total RNA was isolated, and supernatants were collected.
In this scaled algorithm, several arithmetic manipulations are performed that isolate and collect the more multiplicative coefficients they can, and a single scaling coefficient is applied at the end of the calculations.
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