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We computed ω = Ka/ Ks for the complete alignment, ω A = Ka A/ Ks A for the adjacent alignment, and ω 1 = Ka I/ Ks I for the isolated alignment.
The subalignment Ka and Ks estimates were denoted Ka A and Ks A, for the Adjacent alignment, and Ka I and Ks I, for the Isolated alignment.
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To isolate alignment-free issues, in this section we compute k-mers under the assumption that we have completely assembled genomes without error.
They thus isolated the alignment due to dark matter--an alignment so slight that each group surveyed tens of thousands of galaxies to see it.
We also would like to avoid isolated site alignments.
In order to univocally associate a genomic region to each IS, sequencing reads that map equally well to multiple regions of the genome must be discarded: thus, reads from M are subject to further filtering in order to isolate unambiguous alignments.
33 patients (46 knees) had isolated patella re-alignment and 10 patients (12 knees) also had concomitant operative fixation of an associated osteochondral fragment.
Fig. 3 Alignment of isolated and characterized cysteine-rich antifungal proteins from filamentous ascomycetes (a).
Comparisons of computational speed with respect to the literature is somehow difficult to infer since (1) not every work declare computational speed, (2) only isolated blocks of the alignment chain are usually considered (e.g. feature extraction) instead of the entire pipeline, (3) hardware obsolescence.
Areas of dubious alignment were isolated using Gblocks [37] (default settings) and excluded from the analysis.
The detailed sequence differences are summarized in Table S7 and the alignments among isolated cDNAs, predicted ORFs and the corresponding genomic DNA sequences are shown in Additional file 3. The isolated sequence of At1g31420 is 3 bp shorter than the prediction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com