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The four strongly expressing candidates isolated above are promising landing sites.
Non-pooled and pooled RNA isolated above along with a second round of RNA isolation was reverse-transcribed and analyzed separately with TaqMan RT-PCR microRNA assays (Applied Biosystems).
During the rains (June to October) the greater part of the region is flooded to a depth of 3 or more feet (at least 1 metre), leaving the villages and homesteads, which are built on artificially raised land, isolated above the floodwaters.
It concerned the haves and the have-nots of the relatively near future: the have-nots toil in industrial and personal misery on a blighted earth, while the haves live isolated above Earth in a giant space station whose landscapes resemble Beverly Hills.
The Connecticut River appears to have a small (<150 fish) stable population isolated above the Holyoke Dam, and an increasing (895 in 1993, 1,800 in 2003[47]) population in the lower river.
Total RNA samples, as isolated above, were prepared for sequencing using the TruSeq small RNA sample prep kit (Illumina) with multiplexing according to manufacturer's instructions.
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Finally, the effectiveness of PCT in reducing the seismic response of process plant components is proved by three representative case studies: a base isolated above-ground storage tank, a distillation column connected by elastoplastic dampers to the adjacent service frame and an application of non-conventional Tuned Mass Dampers to a support frame.
Alternatively, cells isolated as above were grown in RPMI containing 10% HUS, 180 U rIL-2 and 1% PeSt.
16S rRNA genes were amplified from each genomic DNA sample, isolated as above, by means of a double PCR approach, essentially as described earlier [15].
Taste buds and clusters of taste cells were isolated as above, deposited on coverslips and loaded with fura-2 AM as described previously [34].
CX3CR1+ GC B cells isolated as above were then subjected to chemotaxis to CX3CL1 and migrated cells were characterized by double staining for CD23 and CD27.
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