Sentence examples for isolate membrane from inspiring English sources

The ProteoJET™ membrane protein and cytoplasmic/nuclear protein extraction kits (Fermentas GmbH, St. Leon-Rot, Germany) were used to isolate membrane, cytoplasmic and nuclear proteins.

Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate membrane proteins.

Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific) was used to isolate membrane and cytoplasmic fractions from harvested cells.

To isolate membrane proteins, plant tissue was homogenized in extraction buffer (50 mM Tris, pH 8.5, 150 mM NaCl, 10 mM EDTA, 20% [v/v] glycerol).

In order to increase detection sensitivity of low abundance regulatory proteins using shotgun proteomics techniques, it will likely be helpful to isolate membrane and nuclear proteins separately from cytoplasmic proteins prior to digestions.

Methods are described to isolate membranes from tumor cell lines, solubilize protein and lipid components, and reassemble solubilized components in the form of unilamellar vesicles.

To isolate membranes of N. gonorrhoeae, the strain was plated on GCB plates with the appropriate antibiotic, and (when possible) piliated cells were scraped from the plate and transferred to 3 ml GCBL medium.

To isolate membranes containing LapD (or LapD-sfGFP), 0.5 mg/ml lysozyme was added to thawed cells, which were then lysed by sonication.

As is known, the isolated membrane cytochrome CYP11A1 is able to form oligomers in solution [8].

The p-type polysilicon/gold microthermopiles fabricated on a 2 μm thick thermally isolated membrane showed a sensitivity of 0.94 V/W and a thermal time constant of less than 100 ms.

After three rounds of mutagenesis and screening, the triple mutant E163K/V193M/K170Q yielded a kcat > five times faster than wild type P450 1A2 in steady-state kinetic analysis using either isolated membrane fractions or purified, reconstituted enzymes.