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The organic layer was dried over anhydrous sodium sulphate and concentrated in vacuum to isolate compound 6 as yellow solid.
The organic layer was washed with water followed by brine solution, dried over anhydrous sodium sulphate, filtered and evaporated to isolate compound 5 as a yellow viscous liquid.
Semi-preparative HPLC was used to isolate compound 11 and using direct introduction ESI-HRMS the positive and negative mass spectra were obtained.
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In particular, while solvent extraction will only isolate soluble components, ultrafiltration will only isolate compounds not tightly associated with macromolecules.
To isolate compounds from plants, pressurized liquid extraction (PLE) is frequently applied.
Pantucci, a Xinjiang expert, said the attack appeared to have specifically targeted the Chinese embassy, which is in an isolated compound outside the centre of Bishkek.
The world's largest mass suicide took place on November 18 , 1978inside an isolated compound in Jonestown, Guyana.
MA carried out the interpretation of the isolated compound.
These results indicate that the C. leprosum isolated compound inhibited parasite growth inside the macrophages.
These latter examples showed each isolated compound to exhibit a better MIC than the mixture's.
1H and 13C NMR spectra of the isolated compounds.
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