Isolates with serotypes K1 to K4 were preferentially included in this study; therefore, our isolate collection does not reflect K type frequency in natural populations.
Our isolate collection is biased toward anthrax outbreaks that occurred in the last several decades and towards countries actively engaged in the international exchange of scientific material.
The larger isolate collection [29] was screened by PCR to ascertain the prevalence of genes identified as associated with a particular tissue source by correspondence analysis.
In contrast, our isolate collection is, although smaller in size, a global panel containing isolates associated with different disease manifestations that were collected at different time points in 10 distinct geographic regions on three continents.
MN provided patient care and management and patient isolate collection.
The C. ulcerans isolates were taken from the German Consiliary Laboratory on Diphtheria (NCLoD) isolate collection.
We further tested a Y. pestis isolate collection comprising 2 Antiqua, 6 Medievalis, and 4 Orientalis strains.
The results obtained with the present Tunisian isolate collection suggest that the genetic and phenotypic variability of P. larvae can be larger than that previously estimated.
The 16S rRNA, glpQ, and IGS sequences were unique from all other sequences for these loci in our B. hermsii isolate collection.
Active surveillance also limits case and isolate collection to persons who are residents of the defined surveillance area, allowing for calculation of rates of disease.
All isolates were assigned a code number, preserved in tryptic soy broth (TSB) + 20%% glycerol at – 70 °C and added to the N. gonorrhoeae isolate collection of the reference laboratory.
