Sentence examples for isolate and verify from inspiring English sources

In general, however, any decomposition will ultimately have some interdependence on natural functional boundaries and the ability to isolate and verify the building block.

Plasmids were extracted using alkaline lysis method and further isolated and verified by electrophoresis.

After gene replacement, haploid strain 66132 carrying the hyqΔRI7 allele was isolated and verified by PCR and DNA sequencing analyses.

In this study, we successfully isolated and verified two cyanobacterial strains from two Chinese lakes, Pseudanabaena sp. dqh15 and Planktothricoides raciborskii CHAB 3331, that produce 2-MIB.

The assembled MSP142 fragment (1130 nt) was cloned into the TA vector pCR 2.1 (Invitrogen), producing pCR 2.1-MSP142 FVO, and transformants were isolated and verified by DNA sequencing.

Derived from both A. caulinodans 57100 and 60107R nifA) as parent, hyq merodiploid strains 66205 and 66210, both carrying an upstream in-frame fusion hyqR::uidA+ hyqΔBI operon in tandem with the downstream hyq+ operon, were isolated and verified by both PCR and DNA sequencing analyses (Methods).

Putative positive colonies were cultured and their plasmids isolated and verified by restriction digest.

Individual colonies were selected for growth on YPD plates containing 4% ethanol; the plasmids were isolated, sequenced, and verified for plasmid dependency of the selection by repeating the selection process after transformation into CY1021.

We sequenced VP1 from MCV isolate 344 and verified amino acid residues aspartic acid (D) and arginine (R) at positions 288 and 316 as found in MCV isolates 339 and 162.

This LSP was present in all of the other isolates examined and verified in selected isolates by PCR (Table 3).

The products obtained from the transformants were isolated, analyzed and verified to be ribostamycin derivatives.