The HPLC conditions were as follows: flow rate, 1 ml min−1; solvent A, water containing 0.05% formic acid; and solvent B, MeOH, isocratic system of 78% MeOH.
According to study the high-performance liquid chromatography (HPLC) analyses were carried out on a normal phase column or reverse phase column using a Water 2998 photodiode array detector (260 370 nm) and an isocratic system of elution or on a chiral phase column.
Components were eluted with an isocratic system of 5 mM H2SO4 in H2O.
Samples were treated according to the manufacturer's protocol and HPLC was performed with isocratic system with UV detection (HPLC apparatus ECOM; ECOM, http://www.ecom.cz/).
leaf extract was dissolved in HPLC grade methanol at a concentration of 1.0 mg/mL, filtered through 0.22 μm filter and subjected to HPLC Schimadzu isocratic system equipped with Luna C18 column.
The glucose monomers in each aliquot were determined by high-performance anion exchange chromatography with pulsed amperometric detection (Dionex, UK), using a CarboPac PA20 column with isocratic system at working flow rate of 0.5 ml/min.
Reversed phase HPLC method was developed and validated as per USP monograph using HPLC isocratic system (Waters, Vienna, Austria) for analysis of lorazepam in prepared nanoparticles (USP Monograph Lorazepam, USP30-NF25, 2496).
The sugars, glucose, fructose, and sucrose, were eluted with an isocratic system of 80%acetonitrile/20%% H2O at a flow rate of 1 ml/min and a column temperature of 35°C.
