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Samples were assayed by isocratic high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection.
Retinol was measured using an isocratic high-performance liquid chromatography method as described by Sowell et al. (25).
Serum vitamin C was measured by isocratic high-performance liquid chromatography (HPLC) with electrochemical detection at 650 mV.
Trp and its kynurenine metabolites were determined in liver by our newly developed rapid isocratic high-performance liquid-chromatographic procedure (Badawy and Morgan, 2010).
The concentrations of glucose, pyruvate, acetate, succinate, glycerol, and ethanol were measured by using isocratic high-performance liquid chromatography with refractive Index detection on an Agilent 1100 system (Agilent Technologies, Waldbronn, Germany) equipped with cooled autosampler (5°C).
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A simple, rapid, isocratic reversed-phase high-performance liquid chromatographic method was developed and validated for the analysis of moxonidine and its impurities in tablet formulations.
In the present study an isocratic reversed-phase high-performance liquid chromatography was investigated for the separation of nimodipine and impurities (A, B and C) using statistical experimental design.
After sampling, the cartridges were extracted with acetonitrile, and the DNPH-formaldehyde derivative was analyzed using isocratic reverse-phase high-performance liquid chromatography with an ultraviolet absorption detector operated at 360 nm.
In addition, arginine (n = 1,678) and tryptophan (n = 742) concentrations were determined by isocratic and reverse-phase high-performance liquid chromatography, respectively, as previously described (23, 24).
Urinary noradrenalin was analyzed using the isocratic ion-pair reversed-phase high-performance liquid chromatography method with electrochemical detection [ 31].
Culture supernatants were analyzed for acetate, butyrate, acetone, butanol, ethanol, acetoin, 1,3-propanediol, and glycerol concentrations using an Agilent LC high-performance liquid chromatography system (with 1260 Infinity standard autosampler, isocratic pump, refractive index detector, and in-line vacuum degasser) and Agilent ChemStation software.
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