Amylasemia was tested at admission, and at least twice during hospitalisation, by a specific colorimetric test for the pancreatic subunit according to the manufacturer's instruction (iso-amylase pancreatic, ALIFAX S.p.A Padua, Italy).
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The isotypes of the monoclonal antibodies were determined using Isotyping kit (ISO-2; Sigma) according to the manufacture's instructions.
Total RNA was extracted from the cells by homogenization in Trizol Reagent (Invitrogen cat# 15596-026) and extraction with chloroform followed by precipitation with iso-propyl alcohol as per the manufacturer's instructions.
For analysis of RNA expression, total RNA from the EBs was isolated using RNAStat-60 (Iso-Tex Diagnostics, Friendswood, TX), according to manufacturer's instructions.
The well processed samples were processed according to the manufacturers instructions and then washed according to the times recommended by BS ISO 18929 2012 [18929 2012
A mouse-on-mouse IHC-ISO labeling kit (InnoGenex, San Ramon, CA) was used, according to the manufacturer's instructions and a protocol similar to one described (9 ).
A mouse-on-mouse IHC-ISO labeling kit (InnoGenex, San Ramon, CA, USA) was used, according to the manufacturer's instructions and a published protocol (7 ).
