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Replication of HSV-2 MS or 0ΔNLS in the footpads of vaccine recipients was verified in parallel groups of mice, as follows.
The spermatogenic stage of each tubule used to count spermatogonia and Sertoli cells was verified in parallel cross-sections (3 µm) stained by the periodic acid-Schiff method in order to visualize steps of rat spermiogenesis.
Efficient depletion of the AMA-1 protein was verified in parallel samples (see Additional file 1).
Efficacy of DN-I κB- α transfection was verified in parallel by luciferase assay (see below).
The efficacy of the DNA microarray was verified in a parallel study where pathogen genes and E. coli cells were enumerated using real-time quantitative PCR (qPCR) and standard membrane filter techniques, respectively.
The clones of each cell line were generated and maintained in parallel and α-Syn expression level was verified in the α-Syn expressing clones continuously [37].
The predictions were verified in strains present in the Keio collection of E. coli single-gene knock-out mutants, and a parallel B. subtilis mutant counterpart.
Apoptosis was verified in myocardial cells.
The measurement was verified in January , 2012
They have not been verified in any way.
Results were verified in two independent assays.
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