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A threshold MIC of > =200 μg/ml is used for segregating active B_mols and inactive B_mols.
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The japonica cultivars Nipponbare (NIP), Wuyunjing 7 (WYJ7) and Chunjiang 06 (CJ06) and the indica cultivar Nanjing 06 (NJ06) were used for segregating population construction.
Linkage mapping strategies are limited in detecting loci underlying quantitative traits (QTL) because, commonly, only two extreme parents are used for generating the segregating population, and only a few recombination events are studied (Flint-Garcia et al. 2005).
This is in contrast to current approaches where intergenic polymorphisms are used for scoring a segregating phenotype without the associated gene-related information.
However, not all recombinants produced by self-pollination can be used for fine-mapping, as segregating genotypes within the targeted region are not created when recombinants that are homozygous at both flanking markers are self-pollinated.
In this methodology, one or more pedigrees in which the trait of interest is observed to segregate are used for study.
This segregating population was used for fine mapping of the GNS4 locus.
Segregating plants were used for RNA expression and cosegregation analysis using PCR-based polymorphism.
Fertile plants (homozygous or heterozygous) derived from a segregating population were used for comparison.
Fully informative markers segregating 1 1 1 1 were used for a comparative mapping analysis to reveal discrepancies in the microsatellite marker orders between the two independent parental maps and between them and the consensus map.
Association mapping often is carried out on a diversity panel of lines but also can be used for QTL detection in multiple segregating families (Yu et al. 2008; Liu et al. 2013).
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