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In contrast, when the primary stimulus is too persistent or severe, the ER stress response can lead to irreversible cell damage and programmed death through stimulation of proapoptotic transcription factor growth arrest and DNA damage-inducible gene 153 (Galso53, also called C/EBP homologous protein or CHOP) [ 87, 89– 91].
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The second ingredient, or second level, is passive acknowledgment, when infractions are too persistent to go unnoticed.
He now regrets that he did not do more, fearing that other members regarded him as being too persistent in questioning their leader.
Wealthier relatives from the South can be too persistent in asking about the daily deprivations and oppressive political life in the North.
They worry too much about intruding on the prospect's time or being too persistent out of fear of losing the sale.
2. Don't be too persistent.
Those back-arching 808 kicks are too persistent to be denied, and we can tell you from experience, Miami Bass + Roller Rinks = The Greatest Childhood Experience Ever.
That was OK, but sometimes it was too persistent and they'd go from trying to be seductive to being hostile.
"At some point, I think they decided I was too persistent in asking this question and trying to do my job, and so they arrested me".
Most importantly - don't be too persistent.
Don't be too persistent with the person.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com