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However, this displacement of Rad51 is titrated out by increasing amounts of DNA molecules loaded with S-PCNA before the addition of Srs2.
As summarised in Figure 5, binding of both fusion proteins C595scFv-Fc and C595scFv-Fc-IL2 C595scFv-Fc-IL2 C595scFv-Fc-IL2oncentratisns of soluble titratedide.
We hypothesise that this reduction entails that the E2F inhibitor RB1 is titrated out and that the G1/S-transition checkpoint consequently is overridden, enabling the cancer cells to proliferate.
In viewing of our and other results, although AR proteins with polyQ motifs are conformationally aggregation-prone proteins, ESCs could be poorly adapted to ER stress only if the concentration of chaperones is titrated out as a result of association with accumulating protein aggregation by stress signals, such as androgens.
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Thus, level of cellular uptake in embryonic cells might be not sufficient to ensure a high enough level of the drug within cells or, alternatively, the drug might be titrated out by the high endogenous FKBP12 level existing in embryos that could also, moreover, compete out the FKBP-linked Cre moiety ([42]; personal observations).
In such pathologies, the primary signaling pathways may be titrated out and novel, normally non-physiological pathways may become activated.
Dnmt1 is highly abundant in early Xenopus development and may be sufficient to mediate early repression [ 34], but as development proceeds its levels are titrated out after multiple cell divisions perhaps permitting Lsh to have a more prominent role in specifying repression at discrete loci.
In general, any scheme in which both a first gene product inhibits cellular proliferation and in which this first gene product may be titrated out potently by a second gene product that thereby undoes the inhibition of proliferation, can have the effect of phenotypically switching cells between dormancy and growth.
Otherwise, functional DsbC will be titrated out of the periplasmic space and new DsbC will need to be synthesized.
ICM is titrated care at the physician level, as Crippen points out [ 24]: "Picture your hospital emergency department with a physician on call from home.
Titer was titrated on 3T3 cells.
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