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Thereafter, this was split in four equal parts, and used to infect four replicate 500 L tanks, each containing 20 Atlantic salmon that had not previously been exposed to sea lice.
Thereafter, the pooled lectins were split equally and each fraction was mixed with synovial fluid containing 5 mg protein obtained from 10 pooled RA or SpA samples and made up to 5 ml using Tris buffered saline (0.05 M Tris HCl, pH 7.5, 0.15 M NaCl).
Half of the medium was exchanged on days 5 and 8, and cells were split as needed thereafter.
Thereafter the cells were split into 25 cm-flasks (BD Biosciences) containing medium without G418 supplementation.
When aggregates of polygonal and bizarre epithelioid cells appeared in the background of spindle-shaped cells in the fourth passage, the cells were split and thereafter passaged every week.
Thereafter, the joints were split into two categories with regard to the normalisation of the examination after 4 months of anti-TNF therapies, calculating the probability of observing radiological progression after 2 years with respect to this treatment effect.
Politically, Europe is split.
But SPS is split.
His party is split.
Thai society is split.
The DPJ is split.
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