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In mice, the intensity of the ALK transcript and protein diminishes after birth, reaching minimum levels at 3 weeks of age, and is thereafter maintained at low levels in the adult animal [ 3].
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The sterile molten CMC and xylan media were thereafter maintained at 45 °C in a water bath.
Instantaneously at time t > 0, the temperature of the plate and the species concentration are raised to Tw(> T ∞ ) and Cw(> C ∞ ), respectively, which are thereafter maintained constant, where Tw and Cw are the temperature and species concentration at the wall, respectively, and T∞ and C∞ are the temperature and species concentration far away from the plate, respectively.
Instantaneously at time t > 0, temperature of the plate and species concentration are raised to T w ( > T ∞) and C w ( > C ∞) respectively, which are thereafter maintained constant, where T w, C w are temperature and species concentration at the wall and T ∞, C ∞ are temperature and species concentration far away from the plate respectively.
The pooled cells were thereafter maintained in the RPMI-1640 medescribedribed above and used for all experiments.
The Kb levels were thereafter maintained up to the end of the experiment, 6 8 hrs after inoculation.
The medium was discarded 12 h after seeding and cells were thereafter maintained in serum-free medium supplemented with 10−7 M hydrocortisone hemisuccinate.
Scn1afl/+ mice were thereafter maintained by crossing with C57BL/6J mice.
Antihypertensive treatment was thereafter maintained with sublingual Nifedipine 20 mg.
Differences in survival between the two periods occurred early in the course of the disorder and were thereafter maintained.
The colony was initially established in the Republic of Congo in 1992, and was thereafter maintained in Dijon in vivariums made from Altuglass, containing clayish soil and held at 28±1°C, 80% relative humidity and subjected to a daily cycle of 12 h light and 12 h dark.
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