Sentence examples for is then competent from inspiring English sources

The latter acts as a scaffold for this module, which is then competent to associate with the pre-RC and attract DNA polymerase [ 15, 24- 26].

Once oxidized, TPQ is then competent for a single reductive half-reaction, but in the presence of Zn the resulting aminoquinol cannot be reoxidized to TPQ.

These vesicles are then competent to undergo Ca2+ mediated fusion with the plasma membrane, in response to neuronal stimulation.

The rescue-vector amplicon encoding the 5' and 3' rescue-homology sequences is then transformed into competent cells containing the target BAC and the Red recombination proteins.

The digested mixture is then directly transformed into chemically competent Stratagene XL-10 Gold or NEB 10-beta E. coli cells.

The plasmids were then electroporated into competent BCG and plated on 7H10 media supplemented with OADC and containing 25 µg/ml kanamycin.

Plasmids were then transformed into competent E. coli DH5α cells (LacZ−) and spread onto Luria Broth Agar plates containing 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) and ampicillin.

Briefly, the BtsIA gene was sub-cloned into the pTXB1 vector, which was then transformed into competent E. coli strain with the methylase protection ER2566 pACYC184-Bts1M1M2) containing the T7 RNA polymerase, controlled by the lac operon.

The annealed mix was then transformed into competent JM107 E. coli.

The recombinant plasmids were then transformed into competent E.coli BL21 (DE3) (Promega, Madison, WI).

The ligated products were then transformed into competent DH10B Escherichia coli cells by electroporation.