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One of the possible issues responsible for this is the normalization because in normalized libraries more transcripts are represented and thus the probability of being sequenced is lower for each transcript than in case of non-normalized library.
The leakage area Ainf is calculated as where NL is the normalized leakage and NF is the normalization factor.
where δ(t) is the normalization factor, Y ¯ d ( t ) is the normalized log-melspectral coefficient for feature dimension d and time index t, D is the number of feature dimensions, and Δ is the normalization target.
The first factor is the base points (which are equivalent to Taylor series expanding points) of the non-linear normal modes and the second one is the normalization schemes of the corresponding linear modes.
"What we find most concerning is the normalization of the settlements, including ones deep in the West Bank".
"The only opportunity guaranteed by acceptance of substandard pay and conditions is the normalization of our own mistreatment".
where Z t is the normalization factor.
where K is the normalization factor.
where N nκ is the normalization constant.
Next step is the normalization of FDM.
where z is the normalization factor.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com