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After completion of treatment, patients and physicians were asked to evaluate the efficacy, tolerability, and residual efficacy (continued drug effect after treatment is terminated) using a visual analog scale (VAS) from 0 to 100 points, where 0 = no effect and 100 = very good effect.
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The polymers were terminated using amine-functionalised glass coverslips, enabling the synthetic procedure to be reproducible and scaleable.
The reaction was terminated using 0.5 M EDTA.
All reactions were terminated using 1 µl of 1 M formic acid.
After digestion, the enzymatic reaction was terminated using 5% acetic acid.
The reaction was terminated using 2N H2SO4 and the absorbance was measured at 492 nm in an ELISA reader.
The reaction was terminated using 20 µl of 0.4 M hydrocholoric acid and 1 µl of 330 mM EDTA.
Briefly, RKIP (5 µg) was combined with increasing concentrations of locostatin or DHPE and the reaction was initiated with 100 mM ATP containing 5 µCi of [γ-32P]ATP at 30°C for 10 min. The reaction was terminated using sample buffer (6×) and boiling at 100°C for 5 min. Lysates were separated on SDS-PAGE (12%), transferred to nitrocellulose and visualized by exposing to film.
One or 2.5 µg of RNA was diluted to a total volume of 8 µL and was treated with 1 µL of RQ1 RNase-free DNase (Promega, Madison, WI) and 1 µL of DNase buffer at 37°C in a thermal cycler (Techne, Model TC-312, UK) for 30 min. The reaction was terminated using 1 µL of stop solution and incubating the samples at 65°C for 15 minutes.
Finally, the color reaction was terminated using sulfuric acid.
The reaction was terminated using 2 N HCl.
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