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Here we report the crystal structure of the actin binding domain of plectin and show that this region is sufficient for interaction with F-actin or the cytoplasmic region of integrin α6β4.
There have been reports that expression of the tensin1 PTB domain is sufficient for interaction with DLC1.
Also, deleting the whole tensin SH2 binding motif (440 448), instead of introducing a Y442F point mutation in DLC1, is sufficient for interaction with tensin1 [28].
The LIF2 clone isolated in our screen corresponded to a partial cDNA (aa 204 495), showing that the LIF2 C-terminal region is sufficient for interaction with LHP1.
We show that the LBD fragment is sufficient for interaction with APP.
Analysing only the first 194 amino acids (Upa1ΔC1) showed that this PAM2-containing region of Upa1 is sufficient for interaction.
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Both the AD-Uls1531 897 AD-Uls1531 897–955 frandents were sufficient for interAD-Uls1554 955-Slx5, AD-Uls1554 955teracted with SUMO in the two-hybrid system.
We found that the purified GST-tagged Mmi11 122 amino-terminal fragment bound efficiently to Erh1 (Fig. 1b, c), and that an even shorter fragment containing Mmi1 residues 95 122 was sufficient for interaction (Fig. 1c, d).
The evidence presented in this paper indicates that these video episodes may be sufficient for interaction that is meaningful to a young child.
Similarly deletion of various domains of CYLD showed that amino acids 460 to 592 of CYLD were sufficient for interaction with optineurin (Figure 1B).
Two constructs were made for the fusion of Mpp10p with the Gal4p activating domain, as Lee and Baserga [4] described that amino acids 242 to 498 of Mpp10p are sufficient for interaction with Imp3p.We reproduced the previously observed interactions between Imp3p and Mpp10-5p (aa 1 498) or Mpp10-6p (aa 242 498), but no Mpp10p interaction was revealed with the Imp3Q protein (Figure S2B).
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