To understand the influence of this parameter on the evaluation of population's exposure, the search radius has been successively fixed to three different values (500 m, 1000 m and 2000 m) in four different geographical situations (village, industrial, suburban and inner city).
Each sequence was successively fixed as a "family model sequence" and LEON produced an output list of "non-homologous sequences".
After the 1 mm of lung tissues were successively fixed in 4 % glutaraldehyde and osmic acid, gradient dehydrated, transparentized in dimethylbenzene and embedded with Epon-812, ultrathin sections (50 nm) were prepared by ultramicrotome.
Here, under a constant load of 5 μN and with a fixed centre point, the scanned area was successively set as 7 × 7, 5.6 × 5.6, 4.2 × 4.2, 2.8 × 2.8 and 1.4 × 1.4 μm2.
BAL cells were then successively fixed with 500 μL of 2.5% glutaraldehyde (EMS) in 0.03 M potassium-phosphate buffer, 1% osmium tetroxide in 0.1 M sodium-cacodylate buffer and 0.5% uranyl acetate in 0.05 M maleate buffer (Sigma-Aldrich, Buchs, Switzerland), dehydrated in a graded series of ethanol and embedded in epon resin (Sigma-Aldrich).
Yet, nonstandardized, complex protocols, where the sample is successively pressurized at different fixed lengths, or under different axial loads, make this inflation-extension technique potentially difficult [ 35].
Each new set is successively longer, wider, and heavier.
Linear approximation is successively executed at each sampling time.
The response surface is successively formed in a cumulative manner.
This procedure is successively repeated until fracture occurs.
