Specifically, we used a GFP-tagged version of a temperature-sensitive mutant G protein that is retained in the ER at 39°C, but which can exit the ER and be transported to the cell surface via the Golgi when the temperature is subsequently lowered to 32°C (Presley et al., 1997).
For the +6°C scenario, however, peat accumulation ceased and SOL thickness started to decrease around the year 2060, indicating that the peat production rate was subsequently lower than its decomposition rate.
The temperature was subsequently lowered to 30 °C.
Volatile fatty acid concentrations especially those of acetic acid were decreased (P < 0.05) and those of propionic acid were increased (P < 0.05), thus acetic acid:propionic acid was subsequently lowered (P < 0.05) in cows fed with 5.5% urea-treated rice straw and 2.2% urea + 2.2% calcium hydroxide treated rice straw, respectively.
(Bail was subsequently lowered to $10 million).
Transcript levels were subsequently lowered with an intervening shoulder at day 8 which preceded a further decrease.
The probe was subsequently lowered and low-viscosity silicone oil was applied at the outer edges in order to prevent evaporation from the gel.
Initial exclusion criteria included basal calcitonin concentration >100 ng/l that was subsequently lowered to ≥50 ng/l, as requested by regulators in the USA.
caused significant increase in the levels of the serum enzymes, viz., alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), alkaline phosphatase (ALP) and Bilirubin, which were subsequently lowered by oral administration of 200 mg/kg b.w.
However, after the occurrence of gastrointestinal side effects (loose stools or nausea), the octreotide rate was subsequently lowered to 0.25 mL/kg/h with a resultant increase in the deuterated glucose supplementation to 2,337 mg per liter of fluid, in order to maintain the same infusion rate of deuterated glucose.
