Sentence examples for is subsequently fixed from inspiring English sources

However, if the migrating cell is subsequently fixed and then stained for KIM127, the epitope is detected in the focal zone in keeping with the pattern of immunostaining shown in Figure 1 (data not shown).

Trimmed agar blocks containing fixed cells were subsequently fixed in 1% osmium tetroxide.

The slides were subsequently fixed with methanol and stained with Giemsa (Carl Roth, Germany) as described in the work of Rocha et al. [25].

As mentioned before, apart from adding new features, the COBWEB developments contributed to improve GeoNetwork by polishing features and raising awareness of minor bugs, which were subsequently fixed.

General anesthesia of HCA-7 tumor-bearing mice was induced with inhalation of isoflurane in 40%oxygen/60%0 % nitrogen (gas flow = 1 mL/min), and mice were subsequently fixed in prone position.

Cells were subsequently fixed with 1% formaldehyde, 1% BSA in PBS at room temperature, rinsed in PBS and incubated with a 1 1000 dilution of 2 mg/mL streptavidin-Alexa 488 (Life Technologies, UK) for 30 minutes at 4°C.

The spores were subsequently fixed with 0.2% oxymethylene, washed twice with PBS buffer, and then stained with 30 μg/mL propidium iodide (PI; Beyotime, China) at room temperature for 30 min.

Cells were subsequently fixed in paraformaldeyde at defined time points and subsequently stained with cristal violet.

These Day 5 BMDCs were incubated with ovalbumin (Ova) and were subsequently fixed.

Cells were subsequently fixed with methanol, and nuclear morphology was examined by DAPI as previously described [17].

Cells were subsequently fixed with paraformaldehyde and permeabilized with 100% ice-cold methanol as previously described [31] [33].