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To achieve this, the correlation function is subject to further processing, where the 1/f noise is removed using a smoothing function.
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Out of the total reads, any reads without a perfect 10-nt linker subsequence were directly discarded adjoining the insert, yielding of length 10 nt or longer that were subject to further processing.
The samples were subjected to further processing using high shear and high-pressure homogenizer.
Samples with absorbency at 260/280 and 260/230 nm ratios > 2 were subjected to further processing.
Samples with RNA integrity numbers (RIN) larger than 8 were subjected to further processing and hybridized to the Mouse WG-6 V2 BeadChips (Illumina, San Diego, California) by the UT Southwestern Microarray Core Facility.
The concentration of the extracted RNA was quantified using a Nanodrop ND-1000 Spectrophotometer (NanoDrop, Wilmington, DE) and only samples with absorption ratio 260/280 ≥ 1.8 were subjected to further processing.
The pre-miRNA is then exported to the cytoplasm, where it is subject to further enzymatic processing by Dicer and its partners, producing a ∼22 bp double stranded RNA duplex.
This is subject to further consideration under the charter review.
As shown for numerous substrates, these CTFs or NTFs may then be subject to further proteolytic processing (RIPping) by intramembrane-cleaving proteases (I-CLiPs) [ 78].
Specific designs are subject to further constraints.
All cases are subject to further appeals.
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