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From a theoretical point of view this is supported by Habermasian communication theory, where agreement and common understanding are reached when direct communication is stimulated over time [23].
The catalytic activity of SPAK and OSR1 is stimulated over 100-fold by binding to MO25 isoforms, whereas MST3, MST4 and YSK1 are activated three- to four-fold.
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Briefly, cells were stimulated over-night with an HIV gag pool (NIH AIDS Reagent), CMV, HSV1 + 2, VZV lysates (Virion), EBV lysate (Virusys), an SKSD preparation (Cyanamid Iberica, SA) or TT (gift from Novartis Vaccines).
Combining molecular and biochemical analyses, it was found that bone marrow cell differentiation was stimulated over proliferation on GC-ICEL.
Fresh PBMCs from 5 CLL patients were stimulated over an hour time course with CXCL12, and lysates were generated for IMAC enrichment and LC-MS/MS analysis.
To assess hemispheric differences in the change of the size of the MEP during the experimental tasks, each participant was stimulated over the left and right hemisphere.
Splenocytes were stimulated over night with 5 µg/ml of CTH1 or HIA-MoPn at 37°C/5% CO2 and subsequently incubated for 5 h at 37°C with 10 µg/ml brefeldin A (Sigma-Aldrich, USA) and 0.7 µl/ml monensin/GolgiStop (BD Pharmingen).
The peroneal nerve was stimulated over the dorsum of the foot near the ankle, at 7 centimeters from the recording electrodes.
PBMC samples from four patients with at least four-fold WT1 tetramer response were stimulated over 2 weeks in vitro with WT1 peptidepeptide or CMV pp65 NLV peptide.
Collagen cross-linking has been stimulated over time in vitro [ 8] or articular cartilage surfaces have been degraded in order to stimulate repair in vitro [ 9- 12] as well as in vivo [ 13].
We also noted that PrimPol activity on substrates with template dA was stimulated over 1000-fold by Mn2+ (Table 5 and Table S2), which is even greater than that observed for substrates with template dG.
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