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While the germinal micronucleus is faithfully copied during clonal fission, the macronucleus is reconstituted upon division and is usually composed of a different number of copies of a different subset of the micronucleus genes among daughter cells [ 36, 37].
We have generated dual AAV vectors each containing one half of a large transgene which is reconstituted upon target cell co-infection by either splicing (trans-splicing, TS), homologous recombination (overlapping, OV) or a combination of the two (hybrid).
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The XhoI site was reconstituted upon ligation of the fragment into the vector.
To this end we used an adaptation of the 'split white' system [ 47], in which a functional white gene (w) is reconstituted only upon the correct site-specific integration event.
Neither plasmid alone is able to produce a functional lacZ enzyme, but upon plasmid recombination, a functional lacZ gene is reconstituted and yeast cells turn blue in an X-GAL assay (Figure 2D).
Ca2+ dependence of DNA fragmentation process is based on increased DNA endonuclease activity dependent upon sub-micromolar Ca2+ when the nucleus is reconstituted with NAD+ and ATP [ 39].
Upon binding to the target sequence, a functional nuclease is reconstituted that creates double-stranded breaks in the DNA that are efficiently repaired by non-homologous end joining.
Most biosensors are complementation systems where enzyme or fluorophore activity is reconstituted from non-functional domains secondary to receptor dimerization or conformational changes upon ligand binding [ 1, 2].
This is reconstituted Joseph Campbell: follow your bliss.
A black lipid membrane (BLM) is reconstituted in a classic electrophysiology setup[ 16, 17] and the membrane conductance is measured upon the addition of indoles.
Upon site-specific integration of pKC27_ mw into the landing site, a functional mw gene is reconstituted with the attB/attP site in the intron.
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