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The fusogenic activity of ATL is also observed when purified full-length Drosophila ATL is reconstituted into proteoliposomes, with the fusion of these vesicles detected in vitro (Orso et al., 2009; Liu et al., 2012).
The SoPIP2 1 structure is more sensitive to the temperature changes when it is reconstituted into detergent micelles than when it is reconstituted into lipid membrane systems.
The CD spectrum from SoPIP2 1/POPE∶POPC∶POPS∶Ergosterol mixture does not look very different from the one obtained when the protein is reconstituted into E. coli lipid membranes (Figure 1).
However, when the protein is reconstituted into liposomes attention must be paid to the detergent affinity for liposomes and protein.
To further define whether the ZNF mutants directly affect the membrane deformation activity of pUL31, we used the recently established in vitro system in which full-length pUL34 is reconstituted into artificial membranes; i.e., GUVs (Lorenz et al., 2015).
In addition, when the purified ANT is reconstituted into proteoliposomes high calcium concentrations can induce the formation of non-specific channels [60] and this process is sensitised to [Ca2+] by the addition of purified cyclophilin [61].
Similar(52)
This image can be reconstituted into a paper cheque by the drawer's bank.
And indeed, here was a substance that could, like edible Plasticine, be reconstituted into brilliantly exciting shapes: Hula Hoops and French Fries and Pringles.
The plagioclase may be reconstituted into a more sodium-rich variety (albite), although the original form of the crystal is retained.
Messages (electronic messages, initially) were supposed to travel along this path, to be changed into electric energy by the transmitter, and to be reconstituted into intelligible language by the receiver.
Executives at Advance said the paper and its Web site, NOLA.com, would be reconstituted into a new company, the NOLA Media Group, as a way of adapting to the new ways news was being delivered and consumed in an increasingly digital age.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com