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This shows that, under occlusion, a target is readily lost without occlusion detection.
Only a transient expression of Cas9 is necessary and the carrying plasmid is readily lost using a size-reduced AMA1 variant.
Previous report showed a primary accumulation of Fe in embryo and aleurone layer of rice grain [24], which is readily lost during the polishing process.
It is easy to verify this as O-prenylation, since 68 Da (isoprene) is readily lost in these compounds, as previously described.
Glycerol is an energetically costly solution to freeze resistance as it is readily lost to the environment, likely through the skin and gills [ 63].
However, the plasmid pNDM-BJ01 was found to be unstable and is readily lost if antibiotic pressure is reduced, which implied a limited expansion potential in wild A. lwoffii strain [ 42].
Similar(53)
The results from MS analyses of hydroxycinnamoyl-quinic and hydroxycinnamoyl-isocitric acid show both QA and IA to be readily lost as product ions at m/z 191.
The expected yield increase on the application of inorganic fertilizer is not obtained since these fertilizers are readily lost to leaching due to high rainfall coupled with low activity clays in these soils (Ano and Agwu 2005).
Genes in long pathways should be readily lost from the neo-Y, since selection preventing their loss will be weak.
In summary, even though genes may be readily lost over time from individual organisms, it is extremely improbable for genes to be completely lost from large populations.
However, when L-form growth was selected for in these cells, the plasmid was readily lost; in other words, FtsZ had become dispensable for cell proliferation.
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